| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2089842 | Journal of Microbiological Methods | 2015 | 4 Pages |
Abstract
•We successfully developed a multiplex MLVA format for subtyping of C. difficile.•An excellent concordance between MLVA and TRST typing was found.•We found loci potentially reducible in outbreak investigations of known TRST types.•We identified variable loci providing discriminatory power to the MLVA scheme.
Subtyping of Clostridium difficile is crucial for outbreak investigations. An extended multilocus variable-number tandem-repeat analysis (eMLVA) of 14 variable number tandem repeat (VNTR) loci was validated in multiplex format compatible with a routine typing laboratory and showed excellent concordance with tandem repeat sequence typing (TRST) and high discriminatory power.
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Authors
Mie Birgitte Frid Jensen, Jørgen Engberg, Jonas T. Larsson, Katharina E.P. Olsen, Mia Torpdahl,