Genome distribution and validation of novel microsatellite markers of Fusarium verticillioides and their transferability to other Fusarium species

•A total of 470 microsatellites were found in the genome of F. verticillioides.•A set of 10 polymorphic microsatellite markers are proposed.•Microsatellite markers were cross-transferable to other Fusarium species.

Improved population studies in the fungus Fusarium verticillioides require the development of reliable microsatellite markers. Here we report a set of ten microsatellite loci that can be used for genetic diversity analyses in F. verticillioides, and are equally applicable to other fungi, especially those belonging to the Gibberella fujikuroi clade.