| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2089939 | Journal of Microbiological Methods | 2014 | 7 Pages |
Abstract
•New simple and fast flow cytometry method for yeast species phagocytosis•Different Candida species present different interactions with macrophages.•Quenching effect of PI over Sytox Green fluorescence of the labeled yeast cells
Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells.
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Authors
Catarina Carneiro, Catarina Vaz, Joana Carvalho-Pereira, Célia Pais, Paula Sampaio,