Strain development and optimized fermentation conditions for blood meal using Aspergillus niger and Aspergillus oryzae

•Two mutants were generated using ultra-violet mutagenesis.•The mutants were screened on blood meal fermentation medium.•A series of statistical methods were used to optimize the fermentation conditions.

To hydrolyze blood meal (BM) effectively, two mutants were generated using ultra-violet mutagenesis. Single-factor experiments, the Plackett-Burman technique and response surface methodology were used to optimize the fermentation conditions. This study successfully generated a mutant and innovatively provided important parameters for utilizing BM by fermentation, which could be of industrial value.