| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2089965 | Journal of Microbiological Methods | 2014 | 9 Pages |
•Immunosensor based on electrochemically depositing gold nanoparticles and ionic liquid firstly for detection of S. pullorum.•AuNP with unique electrochemical properties and biocompatibility characteristics can strengthen the antibodies combination and increase the electrochemical signal.•The IL film could overcome the unsatisfying biocompatibility of AuNP and resistance to swelling, retain high enzymatic catalytic activity and facilitate the immobilization.•A crucial assessment regarding implementation of a range of immunosensor to prove AuNP and IL have obvious improvement of reproducibility and stability.
In order to increase the reproducibility and stability of electrochemical immunosensor, which is a key issue for its application and popularization, an accurate and stable immunosensor for rapid detection of Salmonella pullorum (S. pullorum) was proposed in this study. The immunosensor was fabricated by modifying Screen-printed Carbon Electrode (SPCE) with electrodeposited gold nanoparticles (AuNPs), HRP-labeled anti-S. pullorum and ionic liquids (ILs) (AuNP/HRP/IL). AuNPs are electrodeposited on the working electrode surface to increase the amount of antibodies that bind to the electrode and then modified with ILs to protect the antibodies from being inactivated in the test environment and maintain their biological activity and the stability of the detection electrode. The electrochemical characteristics of the stepwise modified electrodes and the detection of S. pullorum were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). As shown in the results of the experiments, AuNPs with unique electrochemical properties as well as biocompatibility characteristics have been proven to be able to strengthen the antibody combination effectively and to increase the electrochemical response signal. In addition, a crucial assessment regarding implementation of stability and reproducibility analysis of a range of immunosensors is provided. We found that application of AuNPs/ILs in the immune modified electrodes showed obvious improvement when compared with other groups. Given their high levels of reproducibility, stability, target specificity and sensitivity, AuNPs and ILs were considered to be excellent elements for electrode modification.
