| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2089991 | Journal of Microbiological Methods | 2014 | 5 Pages |
Abstract
•Our aim was to support the detection of Brucella spp. from lesions in cattle.•We associated the Real-time PCR assay with FTA® Elute cards for diagnosis.•It can be considered as an important diagnostic tool for use in slaughterhouses.
This study reports a real-time PCR assay for the detection of Brucella spp. associated with the FTA® Elute method in lesions observed during sanitary inspections in beef slaughter. Of the total 276 samples, 78 (28.3%) tested positive and 198 (71.7%) negative for Brucella spp. The real-time PCR technique associated with the FTA® Elute method proved to be an important tool for the diagnosis, judgment about and disposal of carcasses and viscera of slaughtered animals.
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Authors
Marília Cristina Sola, Eurione A.G. da Veiga Jardim, Marcius Ribeiro de Freitas, Albenones José de Mesquita,