| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2090027 | Journal of Microbiological Methods | 2013 | 4 Pages |
Abstract
•A novel transformation method for T. acidophilum was developed.•Culturing the cells on Gelrite-based solid medium was improved.•Novobiocin resistant clones were obtained and selected.•No plasmid maintenance was detected.•Variants of the NovRgyrB gene recombined into the chromosomal gyrB gene.
A transformation method yielding up to 104 transformants per μg circular DNA was developed for Thermoplasma acidophilum. The method is based on a natural DNA uptake process in which T. acidophilum cells keep their integrity and turn competent at pH 3.5 and 58 °C. Shuttle vector maintenance could not be detected, since the used NovR gyraseB gene integrated into its chromosomal counterpart by homologous recombination.
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Authors
Erzsébet Baka, Sándor Varga, Cédric Hobel, Roland Wilhelm Knispel, Csaba Fekete, Milán Ivanics, Balázs Kriszt, István Nagy, József Kukolya,