| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2090033 | Journal of Microbiological Methods | 2013 | 4 Pages |
Abstract
•We developed a functionalisation protocol for C. parvum oocysts capture.•We optimised a specific sandwich-type potentiometric detection of C. parvum.•5 × 102C. parvum oocysts per mL were detected in 60 minutes.
We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocysts was potentiometrically detected. This method allowed the detection of 5 × 102Cryptosporidium oocysts per mL in 60 min.
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Authors
Olivier Laczka, Lucy Skillman, William G. Ditcham, Brenton Hamdorf, Danny K.Y. Wong, Peter Bergquist, Anwar Sunna,