| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2090048 | Journal of Microbiological Methods | 2013 | 4 Pages |
Abstract
•We developed a cutting-edge evolution of our method for aac(6′)-Ib-cr detection.•This method combined SYBR Green real-time PCR and pyrosequencing.•Simplicity and rapidity increased cost-effectiveness compared to our previous method.
We previously described a pyrosequencing-based method able to guarantee detection of aac(6′)-Ib-cr by characterizing precisely the single nucleotide polymorphisms leading to Trp102Arg and Asp179Tyr. By gaining in simplicity and cost-effectiveness, through the use of real-time PCR, we propose here a cutting-edge evolution of our existing pyrosequencing method.
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Authors
Thomas Guillard, Nicolas Fontaine, Anne Limelette, Anne-Laure Lebreil, Janick Madoux, Christophe de Champs,