Innovative modifications to Rose Bengal plate test enhance its specificity, sensitivity and predictive value in the diagnosis of brucellosis

•Superagglutination test is more sensitive and partially more specific.•It has high negative and positive predictive values.•It gave minimum false positive results.

Current agglutination tests occasionally yield false results. Superagglutination test reduced false results, had higher sensitivity (95.88%) and negative predictive value (95.83%) than Rose Bengal plate test (RBPT), Standard Tube Agglutination test (STAT), ELISA, and Complement Fixation test and specificity (89.32%) and positive predictive value (89.42%) higher than RBPT and STAT.

Graphical abstractThe novel Superagglutination test developed by us had higher sensitivity, specificity and predictive values but lower false positive and false negative results than RBPT, STAT.Figure optionsDownload full-size imageDownload as PowerPoint slide