Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2090104 | Journal of Microbiological Methods | 2013 | 4 Pages |
Abstract
The study describes a rapid approach for detection of common enteric bacterial pathogens, which involves partial amplification of the 16S rRNA gene by PCR using a colony from selective medium followed by restriction enzyme (RE) digestion using the EcoRI, HindIII and SalI enzymes. On the basis of RE digestion analysis different genera namely, Escherichia, Salmonella, Shigella, Vibrio, Campylobacter, Arcobacter, Yesinia and Listeria were differentiated.
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Authors
J. Vergis, M. Negi, K. Poharkar, D.P. Das, S.V.S. Malik, A. Kumar, S.P. Doijad, S.B. Barbuddhe, D.B. Rawool,