Whole genome amplification of the obligate intracellular pathogen Coxiella burnetii using multiple displacement amplification

•Whole genome amplification employing multiple displacement amplification (MDA)•Fastidious obligate intracellular pathogens like Coxiella burnetii•Technique in detection assays and genomic analysis•For obtaining sufficient genetic material from highly infectious agent•Minimizing repeated culturing and associated biohazard

This study demonstrates that whole genome multiple displacement amplification (MDA) is a promising technique for downstream genomic analysis of fastidious obligate intracellular pathogens such as Coxiella burnetii. The MDA technology can help in obtaining sufficient genetic material from highly infectious agent and thus minimizing repeated culturing and associated biohazard.