Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2090162 | Journal of Microbiological Methods | 2013 | 5 Pages |
Abstract
The Multi Locus Variable Number Tandem Repeat Analysis 16 loci panel (MLVA-16), involving singleplex PCRs and agarose gel electrophoresis, is the standard genotyping method for Brucella spp., used also for the Brucella international online database. We describe an alternative, reliable, high-throughput MLVA-16 protocol using multiplex PCRs and multicolor capillary electrophoresis.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Giuliano Garofolo, Massimo Ancora, Elisabetta Di Giannatale,