A New Standard-Based Polynomial Interpolation (SBPIn) method to address gel-to-gel variability for the comparison of multiple denaturing gradient gel electrophoresis profile matrices

The Standard-Based Polynomial Interpolation (SBPIn) method is a new simple three-step protocol proposed to address common gel-to-gel variations for the comparison of sample profiles across multiple DGGE gels. The advantages of this method include no requirement for additional software or modification of the standard DGGE protocol.

► We present a new method for addressing gel-to-gel variations to compare DGGE gels. ► This simple method does not require additional software nor protocol modifications. ► The method uses custom standards for the alignment and comparison of DGGE profiles. ► Statistical analysis validated the method's robustness handling gel-to-gel variation. ► Profiles run in several gels produce data comparable to profiles run on single gels.