Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2090192 | Journal of Microbiological Methods | 2012 | 4 Pages |
We hybridized biotinylated probes that anneal at multiple locations throughout the Batrachochytrium dendrobatidis (Bd) genome to selectively capture Bd genomic DNA (gDNA) by binding the probe-gDNA complex to streptavidin coated magnetic beads. We then whole genome amplified the captured gDNA. This method extends the usefulness of field-collected swabs for downstream PCR-based genomic applications.
► Batrachochytrium dendrobatidis (Bd) is responsible for massive amphibian declines. ► Low template concentrations and PCR inhibitors limit population studies of Bd. ► We combine magnetic capture hybridization with whole genome amplification. ► We recover Bd genetic material from low template frog skin swabs. ► This method will extend the usefulness of nonconsumptive field-collected swabs.