| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2090216 | Journal of Microbiological Methods | 2012 | 4 Pages |
Abstract
We have developed a multiplex PCR assay for rapid identification and differentiation of cultures for Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex. The assay is valuable for use in clinical and veterinary laboratories, and in a deployable laboratory during outbreaks.
► A multiplex PCR to identify and differentiate burkholderial species was developed. ► Primers were designed based on hypothetical genes of B. pseudomallei. ► This PCR assay is cost-effective, rapid and easy to interpret. ► This assay is valuable for use in clinical and veterinary laboratories.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Seng Fook Koh, Sun Tee Tay, Rasana Sermswan, Surasakdi Wongratanacheewin, Kek Heng Chua, Savithri D. Puthucheary,