Article ID Journal Published Year Pages File Type
2090308 Journal of Microbiological Methods 2011 4 Pages PDF
Abstract

A novel multiplex PCR was developed which targeted virulence genes associated with the major clonal complexes (CCs) of healthcare- and community-associated methicillin-resistant Staphylococcus aureus (MRSA) in the USA. Most isolates (40/66) were identified as CC 5, while remaining isolates represented CCs 1, 8, 30, 45, 59, 133, and five isolates were not S. aureus.

Research highlights► Virulence genes were targeted to develop a multiplex PCR for detecting MRSA clonal complexes. ► The M-PCR detected 34 stains in the collection of 82 reference strains and nasal isolates studied. ► A majority of isolates were CC5 common to the northeastern USA, where the study was conducted. ► The M-PCR exhibited a similar discriminatory power as MLST, and should be further studied using a larger sample.

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