Article ID Journal Published Year Pages File Type
2090450 Journal of Microbiological Methods 2010 4 Pages PDF
Abstract

The utilization of quantitative PCR (qPCR) approaches such as MPN-qPCR and real-time-qPCR for in situ assessment of functional genes yields substantial quantitative and qualitative differences. We show this by targeting the alkB gene related to biodegradation of aliphatic alkanes in a set of environmental samples with differing hydrocarbon content.

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Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology
Preview
A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil
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