Article ID Journal Published Year Pages File Type
2090470 Journal of Microbiological Methods 2011 6 Pages PDF
Abstract

The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for the differentiation between viable and dead bacteria.

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Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology
Preview
Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua
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