| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2090701 | Journal of Microbiological Methods | 2009 | 4 Pages |
Abstract
In situ Reverse Transcriptase PCR (in situ RT-PCR) can amplify mRNA and localize gene expression in cells. However, this method is not feasible in fungi as the thick fungal cell wall constitutes a barrier to this procedure. We developed a two step in situ RT-PCR procedure which enabled the detection and localization of Candida tropicalis mRNA expression in formalin-fixed, paraffin-embedded (FFPE) mouse kidney sections. This in situ hybridization study revealed the first direct evidence for deposition of Candida tropicalis secreted aspartic proteinase 2 (CtSAP2) in the tip of pseudohyphae and its involvement in acute systemic candidiasis. We conclude that in situ RT-PCR can be successfully applied to FFPE tissues and will offer new perspectives in studying gene expression in Candida species.
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Authors
Voon Chen Yong, Khang Wei Ong, Shiran Mohd Sidik, Rozita Rosli, Pei Pei Chong,