| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2090702 | Journal of Microbiological Methods | 2009 | 4 Pages |
Abstract
Appropriate detachment treatments are required to analyze prokaryotes associated with streambed sediments by flow cytometry. Using our previously optimized protocol, two groups of cells exhibiting different nucleic acid contents were easily detectable. However, the Nucleic Acid Double Staining assay proved that detachment procedures negatively affect the cell membrane integrity.
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Authors
Stefano Amalfitano, Stefano Fazi, Alberto Puddu,