| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2090842 | Journal of Microbiological Methods | 2009 | 4 Pages |
Abstract
We describe a novel ultrasensitive cell-based immunocytotoxicity assay for detecting Clostridium difficile toxin A and B. The assay is simple to perform with a turnaround time of approximately 3 h . It is particularly sensitive in detecting TcdA at a level less then 1 pg/ml. Using this assay, we were able to detect the presence of C. difficile toxins in the fecal and serum specimens of experimentally infected piglets.
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Authors
Xiangyun He, Jufang Wang, Jennifer Steele, Xingmin Sun, Weijia Nie, Saul Tzipori, Hanping Feng,