Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2090906 | Journal of Microbiological Methods | 2008 | 4 Pages |
Abstract
In the present study, a polyclonal antibody was raised against the Gram-negative marine bacterium, Alteromonas sp. A microplate-based immunofluorescence bioassay using QD strepavidin conjugates was developed for quantifying putative Alteromonas sp. cells located on the surfaces of a marine harpacticoid copepod, Microarthridion littorale. The number of attached Alteromonas sp. was estimated to be 102 ± 8 CFU using this method. The QD approach, coupled to a microplate assay can potentially provide an efficient and accurate method for rapidly detecting multiple bacteria species attached to small invertebrate animals because of their unique excitation and emission characteristics.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Erin M. Beckman, Tomohiro Kawaguchi, G. Thomas Chandler, Alan W. Decho,