Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2090947 | Journal of Microbiological Methods | 2009 | 4 Pages |
Abstract
Real Time-PCR (RT-PCR) and high resolution melt (HRM) analyses were used for rapid typing of genes encoding components of the pertussis acellular vaccine, namely prn, ptxA, fhaB, fim2 and fim3. The length polymorphisms in prn were detected by RT-PCR followed by HRM; single nucleotide polymorphisms in prn and other genes were detected by hairpin primer RT-PCR. These rapid methods are suitable for large-scale studies of vaccine-driven evolution of Bordetella pertussis.
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Authors
Wai-Fong Chan, Ram P. Maharjan, Peter R. Reeves, Vitali Sintchenko, Gwendolyn L. Gilbert, Ruiting Lan,