Article ID Journal Published Year Pages File Type
2090971 Journal of Microbiological Methods 2008 4 Pages PDF
Abstract

Modifying the Site-directed, Ligase-Independent Mutagenesis (SLIM) protocol from a single reaction mode to a two-reaction mode enables highly efficient mutagenesis of plasmid constructs that exceed 8kb. This modified approach reduces the complexity of the PCR step and is optimised for generation of heteroduplexes from long PCR products. The two-reaction mode SLIM has 92% efficiency.

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Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology
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