| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2091027 | Journal of Microbiological Methods | 2008 | 6 Pages |
Abstract
To cure Escherichia coli for plasmids derived from the ColE1 replicon advantage is taken of the fact that maintenance of this replicon requires a wild-type allele of polA, encoding DNA polymerase I. Curing is achieved by cotransduction of a mutant polA allele with metE::Tn10, fadAB::Tn10 or other transposon insertions near polA. Reciprocal transduction to Met+ Pol+ or to Fad+ Pol+ ensures reestablishment of the original genotype except for loss of the plasmid. A set of useful bacterial strains is provided.
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Authors
Bjarne Hove-Jensen,