| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2091049 | Journal of Microbiological Methods | 2008 | 5 Pages |
Abstract
Purification of the obligate intracellular bacterium Coxiella burnetii requires physical disruption of infected cells. Here we describe a gentle and safe digitonin lysis procedure to release C. burnetii from infected cells. The purity, yield, and infectivity of digitonin-prepped organisms are comparable to that of organisms purified using cell lysis by sonication.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Diane C. Cockrell, Paul A. Beare, Elizabeth R. Fischer, Dale Howe, Robert. A. Heinzen,