Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2091111 | Journal of Microbiological Methods | 2007 | 9 Pages |
Abstract
Alternaria tenuissima is a fungus widely present in the environment and causes diseases in plants and humans in the world. In this study, we constructed an A. tenuissima cDNA expression library in a centromeric yeast vector that allows the isolation of functional cDNA sequences from this environmental and pathogenic fungus. Through a genetic approach we have isolated and functionally characterized the cDNA sequences encoding the MAP kinase (MAPK) Hog1p and the MAPK kinase Pbs2p of A. tenuissima. AtHOG1 cDNA encodes a protein of 355 amino acids, while AtPBS2 cDNA encodes a protein of 683 amino acids.
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Authors
Fei Feng, Dewen Qiu, Linghuo Jiang,