| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2091207 | Journal of Microbiological Methods | 2006 | 4 Pages |
Abstract
A CARD–FISH protocol was developed and applied to analyse surface-associated bacteria on the marine algae Ulva lactuca, Delisea pulchra, Corallina officinalis, Amphiroa anceps, Porphyra sp. and Sargassum linearifolium. The combination of Alexa546-labelled tyramide as the reporter molecule with SYBR Green II counterstain allowed for superior detection of the hybridised probe fluorescence against plant tissue from which pigment autofluorescence has been reduced.
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Authors
Niina A. Tujula, Carola Holmström, Marc Mußmann, Rudolf Amann, Staffan Kjelleberg, Gregory R. Crocetti,