| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2091211 | Journal of Microbiological Methods | 2006 | 5 Pages |
Abstract
A new means of rapidly and simultaneously counting viable phylogenetically different bacteria was developed. The cyanine dimer dye, BOBO-3 that selectively stains bacteria with damaged membranes were used to evaluate bacterial viability based on membrane integrity. Viable Enterobacteriaceae and Pseudomonas spp. could be selectively detected within three hours using multicolor fluorescence in situ hybridization (FISH) following BOBO-3 staining (BOBO3-FISH).
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Authors
Akiko Kitaguchi, Nobuyasu Yamaguchi, Masao Nasu,