Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2091213 | Journal of Microbiological Methods | 2006 | 5 Pages |
Abstract
A real-time PCR assay targeting the highly specific erm34 sequence of Bacillus clausii DNA was developed and optimized. The quantitative assay showed a sensitivity level of 102 CFU/μl of sample. The method may represent a useful tool for monitoring the role of B. clausii as probiotic in vivo.
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Authors
Mario Perotti, Nicasio Mancini, Annalisa Cavallero, Silvia Carletti, Filippo Canducci, Roberto Burioni, Massimo Clementi,