Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2091270 | Journal of Microbiological Methods | 2006 | 4 Pages |
Abstract
The expressional levels of genes in swarmer cells can be determined by a simple method using X-gal-containing semisolid agars and lacZ-fusion transcription reporter strains of the genes concerned. However, X-gal alone inhibited the swarming of Vibrio, regardless of their ability to digest X-gal. Moreover, X-gal inhibited the growth of V. vulnificus containing functional lacZ. These effects of X-gal itself should be carefully considered when trying to determine the expression levels of genes in swarming cells using X-gal-containing semisolid agar.
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Authors
Moon-Young Kim, Ra-Young Park, Young-Hoon Bai, Yoon-Young Chung, Choon-Mee Kim, Soo-Young Kim, Joon-Haeng Rhee, Sung-Heui Shin,