| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2091271 | Journal of Microbiological Methods | 2006 | 4 Pages |
Abstract
A rapid method combining the use of protoplasts and electroporation was developed to transform recalcitrant wild strains of Bacillus subtilis. The method described here allows transformation with both replicative and integrative plasmids, as well as with chromosomal DNA, and provides a valuable tool for molecular genetic analysis of interesting Bacillus strains, which are hard to transform by conventional methods.
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Authors
Diego Romero, Alejandro Pérez-García, Jan-Willem Veening, Antonio de Vicente, Oscar P. Kuipers,