Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2091356 | Journal of Microbiological Methods | 2006 | 5 Pages |
Abstract
The detection of low abundance mRNA and/or GC-rich targets is very difficult using real-time PCR, often requiring laborious optimization procedures. This work shows that formamide is a useful PCR additive, increasing the sensitivity and specificity of SYBR Green real-time PCR to detect low abundance mycobacterial RNA from infected samples.
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Authors
Manuela Grassi, Elisabetta Volpe, Vittorio Colizzi, Francesca Mariani,