Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2091428 | Journal of Microbiological Methods | 2006 | 7 Pages |
Abstract
A semi-selective medium, PA 20, was developed for the isolation and enumeration of Pantoea ananatis from plant material, specifically from onion seed. The medium has a pH of 8.0 and contains NH4H2PO4, K2HPO4, magnesium sulphate, NaCl, d (+) arabitol, crystal violet, bromothymol blue and thallium nitrate. All P. ananatis strains from a variety of hosts produced characteristic yellow colonies in 6–7 days at 25 °C. Plating efficiencies on PA 20 in comparison to nutrient agar ranged from 92 to 112%. Recovery from naturally infested and artificially contaminated onion seed was high, with an almost total reduction of saprophytes.
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Authors
Teresa Goszczynska, Stephanus N. Venter, Teresa A. Coutinho,