| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2091480 | Journal of Microbiological Methods | 2012 | 4 Pages |
Abstract
In this work a simple and novel method to evaluate the efficacy of compounds on intracellular Leishmania amastigotes by using a fluorometric assay has been developed. The new method is sensitive, easy to perform and scalable for high throughput and therefore it could be validated for screening of new anti-leishmanial agents.
► This new fluorometric method is highly sensitive and easy to perform. ► It is suitable for quantification of intracellular viable Leishmania parasites. ► The fluorometric assay is scalable for high throughput. ► It could be validated for screening of new antileishmanial agents.
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Authors
Pablo Bilbao-Ramos, Sergio Sifontes-Rodríguez, María Auxiliadora Dea-Ayuela, Francisco Bolás-Fernández,