| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2091487 | Journal of Microbiological Methods | 2012 | 4 Pages |
Abstract
Application of pyrosequencing of six Salmonella-specific genes as a rapid Salmonella identification method was tested. Primers for hns, hisJ and hilA had non-specific reactions with non-Salmonella strains. Primers for invA, iroB and fimY had specific PCR products and pyrosequences of Salmonella, suggesting that they were suitable for Salmonella rapid identification.
► Pyrosequencing identification of Salmonella was studied. ► Six previous Salmonella identification genes were assessed. ► Only invA, iroB and fimY were suitable for Salmonella rapid identification. ► It required less than 3 h to have pyrosequencing identification results. ► Pyosequencing provides another rapid identification method with high specificity.
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Authors
Xiujuan Li, Fang Yang, Weili Gao, Hongmei Song, Huifang Tian, Baohong Xu,