Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2091672 | Journal of Microbiological Methods | 2008 | 4 Pages |
Abstract
The results from bacterial strain recovery efforts following hurricanes Katrina and Rita are reported. Over 90% of strains frozen in 10% skim milk were recovered whereas various recovery rates were observed for glycerol-stored stocks (56% and 94% of Escherichia coli, depending upon the laboratory). These observations led to a viability comparison of Streptococcus pyogenes, Campylobacter jejuni, Borrelia burgdorferi, Salmonella enterica subsp. Typhimurium, Pseudomonas aeruginosa and E. coli strains stored in glycerol or skim milk. In all bacteria examined, 10% skim milk resulted in significantly longer viability after thawing than 15% glycerol solutions currently used in most laboratories.
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Authors
William L. Cody, James W. Wilson, David R. Hendrixson, Kevin S. McIver, Kayla E. Hagman, C.M. Ott, Cheryl A. Nickerson, Michael J. Schurr,