| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2091940 | Journal of Microbiological Methods | 2006 | 4 Pages |
Abstract
The iron-deficient culture supernatant of a soil bacterial strain identified as Erwinia sp. was analyzed using a new high-resolution polyamide thin layer chromatography (TLC) and a silica TLC. The results showed both TLC methods were very effective for separating simple catechol compounds such as 2,3-dihydroxybenzoic acid (2,3-DHBA) and catechol. However, in the analysis of more complicated catechol compounds or true catechol-type siderophores (conjugates of 2,3-DHBA and amino acids), the polyamide TLC had the higher resolution. Polyamide TLC analysis showed that strain S1 produced three distinct catechol-type siderophores.
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Authors
Xiaojun Xie, Jingguo Wang, Hongli Yuan,