| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2093884 | Stem Cell Research | 2016 | 3 Pages |
Abstract
Chronic myeloid leukemia (CML) is a clonal malignancy initiated by the occurrence of a t (9;22) translocation, generating Ph1 chromosome and BCR-ABL oncogene in a primitive hematopoietic stem cell (HSC). The resistance of HSC to targeted therapies using tyrosine kinase inhibitors remains a major obstacle towards the cure. We have generated an iPSC line from a patient with CML using leukemic CD34 + cells cryopreserved at diagnosis. Ph1 + CML cells were reprogrammed by non-integrative viral transduction. These iPSCs harboured Ph1 chromosome and expressed pluripotency hallmarks as well as BCR-ABL. Teratoma assays revealed normal differentiation after injection in immunodeficient mice.
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Authors
G. Telliam, O. Féraud, F. Griscelli, P. Opolon, D. Divers, A. Bennaceur-Griscelli, A.G. Turhan,