Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
21010 | Journal of Bioscience and Bioengineering | 2011 | 4 Pages |
Abstract
AFUEI, an elastase inhibitor produced by Aspergillus fumigatus strongly inhibits the elastolytic activity of A. fumigatus etc. To purify AFUEI, we constructed a strain that overproduces AFUEI by introducing the gene encoding AFUEI (Genbank accession no. AB546725) under control of the amyB promoter into the heterologous host Aspergillus oryzae. A. oryzae TF-4 displayed strong elastase inhibitory activity and produced considerably more AFUEI than that of A. fumigatus. Furthermore, AFUEI could be purified using culture broth and single ultrafiltration (UF) treatment, allowing for the effective production of AFUEI for use in clinical trials.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Nobuo Yamashita, Yumiko Komori, Yoshiyuki Okumura, Kei-ichi Uchiya, Takeshi Matsui, Akira Nishimura, Kenji Ogawa, Toshiaki Nikai,