| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 21048 | Journal of Bioscience and Bioengineering | 2011 | 4 Pages |
Abstract
Novel vectors (pLCHS-hph and pChG-bar) containing expression unit driving hph as a selectable marker gene by chitin synthase gene promoter were constructed for Lentinula edodes transformation. Expression of the hph gene in random selected transformants was confirmed by RT-PCR method. Thus, both vectors are useful for L. edodes transformation.
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Physical Sciences and Engineering
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Authors
Toshitsugu Sato, Kumiko Okawa, Tatsuya Hirano,