Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
21088 | Journal of Bioscience and Bioengineering | 2010 | 4 Pages |
Abstract
Using yeast genetic complementation, we show here that Rhodobacter sphaeroidesl-lactate dehydrogenase can functionally replace flavocytochrome b2 in Saccharomyces cerevisiae, only when a matrix-targeting signal of flavocytochrome b2 is fused with the bacterial enzyme. The recombinant l-lactate dehydrogenase may add alternative route of mitochondrial electron transport other than flavocytochrome b2.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Hiroaki Sakai, Kazuhiro Nagahama, Hisataka Taguchi, Takashi Akamatsu, Shigeru Morimura, Kenji Kida, Masayoshi Matsuoka,