Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
21106 | Journal of Bioscience and Bioengineering | 2010 | 4 Pages |
Abstract
In static culture of rabbit chondrocytes in collagen gel, the direct measurement of dissolved oxygen (DO) concentration revealed that the DO level at the top surface of gel decreased due to an increase in overall cell density with elapsed time. The local cell density at the top surface on day 21 was 5.7 × 107 cells/cm3, being 11 times that at the bottom of gel. This heterogeneity of cell distribution in the gel was considered to occur by limitation of oxygen supply into a deeper part of the gel. In shaking culture using a dish with gas-permeable film, the DO level was enhanced inside the gel and the overall cell density in the gel was achieved to be 2.9 times that in the static culture.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Masahiro Kino-oka, Shogo Kagita, Masrina Mohd Nadzir, Hirofumi Inoue, Katsura Sugawara, Masahito Taya,