| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 21191 | Journal of Bioscience and Bioengineering | 2011 | 4 Pages |
Abstract
We isolated a Chlamydomonas gene encoding putative γ-glutamyl kinase (GK), an enzyme that catalyzes the first step of proline biosynthesis. Using an Escherichia coli auxotroph and a purified recombinant protein, we show that Chlamydomonas GK is a functional GK. The sensitivity of this kinase to feedback inhibition by proline was lower than in that of microbial GKs previously reported.
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Authors
Keita Miyoshi, Hideyuki Matsuura, Takao Kumon, Keita Matsuda, Tomoaki Yoshikawa, Takeshi Bamba, Kazuo Harada, Kazumasa Hirata,