Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
21363 | Journal of Bioscience and Bioengineering | 2010 | 4 Pages |
Abstract
A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Masamichi Kajita, Masaki Magari, Kagefumi Todo, Naoki Kanayama, Hitoshi Ohmori,