| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 21430 | Journal of Bioscience and Bioengineering | 2009 | 4 Pages |
Abstract
We developed a novel in vitro antibody (Ab) generation system using a hypermutating chicken B cell line (DT40-SW). We suppressed the expression of the Pax5 transcription factor by targeted disruption of the gene to increase Ab production in isolated clones and produce the desired Abs. This single genetic manipulation resulted in a significant enhancement of Ab production without significantly affecting maximum cell density.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Masaki Magari, Takahiro Aya, Mika Ikeda, Kagefumi Todo, Naoki Kanayama, Hitoshi Ohmori,