| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 21452 | Journal of Bioscience and Bioengineering | 2011 | 4 Pages |
Abstract
An efficient method for the direct extraction of yeast genomic DNA from agave must was developed. The optimized protocol, which was based on silica-adsorption of DNA on microcolumns, included an enzymatic cell wall degradation step followed by prolonged lysis with hot detergent. The resulting extracts were suitable templates for subsequent qPCR assays that quantified mixed yeast populations in artisan Mexican mezcal fermentations.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Manuel Reinhart Kirchmayr, Luis Eduardo Segura-Garcia, Ericka Patricia Flores-Berrios, Anne Gschaedler,