Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
21622 | Journal of Bioscience and Bioengineering | 2011 | 5 Pages |
Elicitation of Plumbago indica hairy roots with yeast carbohydrate fraction, chitosan, manganese chloride, copper chloride and methyl jasmonate exhibited significant elevation (~ 1.2 to 2 fold) of plumbagin production in shake flask culture as compared with control. Chitosan and methyl jasmonate elicitation also caused simultaneous plumbagin leaching into culture media. Three days’ exposure of chitosan (200 mg l−1) and methyl jasmonate (80 μM) together synergized total plumbagin yield to its maximum 11.96 ± 0.76 mg g−l DW in shake flask culture. In bioreactor cultivation, a significant raise in fresh root biomass was recorded on day 20 as compared with control shake flask culture. Three days’ exposure of chitosan (200 mg l−1) and methyl jasmonate (80 μM) with 20 days old bioreactor-culture significantly improved total plumbagin production to 13.16 ± 1.72 mg g−l DW with simultaneous plumbagin leaching into bioreactor media.