| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 21687 | Journal of Bioscience and Bioengineering | 2009 | 4 Pages |
Abstract
The mustard trypsin inhibitor 2, MTI2, was expressed in Escherichia coli. A specific procedure for its production and purification is described. The recombinant protein was recovered by protein extraction from the insoluble fraction, then renatured and purified by ion exchange and gel filtration chromatography. Finally, the inhibitory activity against trypsin was also determined.
Keywords
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Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Alessandra Stefan, Luisa Ugolini, Elena Martelli, Sandro Palmieri, Alejandro Hochkoeppler,